The fight for independence from the colonial yoke gained momentum in the early 20th century. Anti-colonial sentiment reached its peak in the interwar period as a result of the mass movements initiated by Gandhi, and his ideas of “non-violence,” ‘boycott’ and ‘swadeshi’ had a significant impact on the minds of the native population. This essay examines the impact of Gandhian ideology on the development of public health infrastructure in Bombay city during the interwar period. It highlights the contribution of the medical professionals and students in Bombay, challenging the colonial authorities and constructing a national identity through the lens of public health infrastructure.